Techniques in Molecular Biology

1. High Performance Liquid Chromatography of Proteins and Nucleic Acids. - 2. Analytical Electrophoretic Techniques in Protein Chemistry. - 3. Protein Blotting. - 4. The Production and Isolation of Peptides for Sequence Analysis. - 5. Protein and Peptide Sequence Determination. - 6. The Extraction and Fractionation of RNA. - 7. Protein Synthesis In Vitro. - 8. Labelling DNA In Vitro Nick Translation. - 9. Synthesis of cDNA for Molecular Cloning. - 10. Plasmid Isolation. - 11. The Use of Restriction Endonucleases and T4 DNA Ligase. - 12. Molecular Cloning of cDNA: Bacterial Transformation and Screening of Transformants. - 13. The Use of Minicells and Maxicells to Detect the Expression of Cloned Genes. - 14. Gel Electrophoresis of DNA. - 15. Detection of Specific DNA Sequences The Southern Blot. - 16. The Determination of DNA Sequences. - 17. The Use of Cosmids as Cloning Vehicles. - Notes on Contributors. Language: English